A Source can be any population, organism or part of an organism which is used as part of an experiment. The name(s) of the species known to be in this Source For example "wild population", "laboratory animal", "cell culture", "soil sample" etc. Where is this Source to be found? Additional metadata A SamplingProtocol describes the method used for obtaining a Sample from Source A Sample is some portion of a Source which has been isolated for investigation Where did this Sample come from? How was this Sample obtained? When was this Sample acquired ? Who carried out the SamplingProtocol ? What sort of material is this sample? For example 'blood', 'frozen tissue', 'whole organism' etc. How much material is in this Sample ? Additional metadata A TreatmentProtocol describes a process used to differentiate biological materials during an experiment, for example incubation, drug doses, stavation and so on. A TreatedSample is a Sample or TreatedSample that has had something done to it, for example being exposed to a stress or environmental change, or being combined with other Samples or TreatedSamples. The treatment that was applied is described by the TreatmentProtocol to which it is linked. Which Sample(s) does this TreatedSample come from? A TreatedSample can be linked to one or more Sampless to construct a chain of treatments Which other TreatedSample(s) does this TreatedSample come from? A TreatedSample can be linked to one or more other TreatedSamples to construct a chain of treatments this is the pre-comment for the 'Treatment Details' group this is the post-comment for the 'Treatment Details' group What treatment was applied to produce this TreatedSample? When was this action done? Who did this action? Additional metadata A PreparationProtocol describes a process used to prepare a Sample or TreatedSample for use in an Assay, for example dye-labelling, cell separation, and so on. A PreparedSample is a Sample or TreatedSample which has been prepared for use in one or more Assays. Which Sample(s) does this PreparedSample come from? A PreparedSample can be linked to one or more Samples (unless it has already been linked to some TreatedSamples) Which TreatedSample(s) does this PreparedSample come from? How was this PreparationProtocol prepared for for assaying? When was this action done? Who did this action? Additional metadata AssayEquipment represents the collection of hardware and/or software that was used to perform an Assay Additional metadata An AnalysisProtocol describes the process by which the results in an AssayDataSet were obtained from an Assay or from another AssayDataSet Additional metadata An AssayDataSet is a collection of results obtained by performing an Assay on one more PreparedSamples, or by performing an analysis on one or more other AssayDataSets. Additional metadata If this AssayDataSet represents data that were generated by analysis of an Assay, then these fields should be used. From which Assay(s) was this data acquired? How was the data generated? When was this data acquired? Who performed this action? If this AssayDataSet represents data that were derived from some AssayDataSet, then these fields should be used. Which other AssayDataSet(s) were used to derive this one? How was this data derived? When was this data generated? Who performed this action? A AssayProtocol describes a process used to perform an Assay. An Assay is any process by which one or more observations are made to determine the biomolecular properties of one or more PreparedSamples either by a simple test, such as measuring pH or taking a photgraph though a microscope, or by the application of a high-throughput mechanism such a microarray analysis or mass spectrometry. What AssayEquipment was used for this Assay? An Assay must be linked to an AssayEquipment which describes the hardware and software used in that Assay Which PreparedSample(s) were examined in this Assay? An Assay must be linked to at least one PreparedSample Which AssayProtocol was followed for this Assay? An Assay must be linked to the AssayProtocol which describes how it was performed When was this assay carried out? Who did the action? Additional metadata A Study is a set of related Assays A Study can be linked any number of Assays Additional metadata An Investigation is a collection of related Studies which share some common goal An Investigation can be linked any number of Studies Additional metadata